Mass Spectrometry

Mass spectrometers determine the mass/charge and abundance of ionised analytes. Mass spectrometers consist of three basic components, a source, and analyser and a detector. Ions are introduced into the mass spectrometer via the source. The internal environment is kept under constant vaccuum and ions. The analyser separates analytes by mass/charge ratio (m/z) using electromagnets. The detector uses electron multipliers to detects the analytes after separation. The detectors are able to measure the abundance of analytes. The resultant data is an m/z and an intensity value for each analyte. The speed and resolution is dependent on the type of analyser used. There are numerous types of analyser and they are often used in series. There are therefore many configurations of mass spectrometers. It is important to select the right type of mass spectrometer for the type of analyses required.

Equipment locally avaialble

Mass spectrometers available in local core facilities or other proteomics research labs

AB SCIEX TripleTOF 6600

"TripleTOF technology unleashes sensitivity, speed, and productivity in a single multifaceted platform to help you dig deeper into complex samples."

CSIR Proteomics and IDM Proteomics

Thermo Fisher Q Exactive

Hybrid Quadrupole-Orbitrap Mass Spectrometer. This benchtop LC-MS/MS system combines quadruple precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver exceptional performance and versatility

CPGR and IDM Proteomics

Thermo Fisher LTQ Velos

Combines the proven mass accuracyand ultra-high resolution of the Orbitrapmass analyzer, with the increased sensitivi-ty and improved cycle time of the LTQ Velos

CAF

Thermo Fisher Orbitrap Fusion

Orbitrap Fusion™ Tribrid™ Mass Spectrometer. This instrument combines the best of quadrupole, ion trap and Orbitrap mass analysis in a revolutionary Tribrid architecture to provide unprecedented depth of analysis and ease of use.

CAF

Spectral Viewers

Spectral viewer software, for visualising the data produced by mass spectrometry

Skyline

Targeted Analysis software that allows you to interrogate specta

IDM Proteomics, CPGR, CAF and CSIR Proteomics

R for Proteomics

R package for viewing and analysing proteomics data

IDM Proteomics

Xcalibur

Thermo Fisher Mass Spectrometry Spectrum Analyser

IDM Proteomics, CPGR and CAF

MaxQuant Viewer

Spectrum Analyser for MaxQuant generated results

IDM Proteomics, CPGR, CAF and CSIR Proteomics

Quality Control

Quality control software is used to ensure that the mass spectrometry analysis was successful

Panorama

Integrates into Skyline pipeline allowing aggregation of data, and longitudinal analysis

Quameter

MSQC software to compute diverse metrics from experimental LC–MS/MS data, enabling quality analysis and quality control (QA/QC) of proteomics instrumentation.

IDM Proteomics

Biognosys Quic

"Proteomics MS monitoring software for DDA, DIA, PRM, and MRM measurements from various MS platforms."

CPGR