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Past Training

Bioinformatics

Microbiome Metabarcoding Data Analysis

ABOUT

The South African Institute for Aquatic Biodiversity (SAIAB), in collaboration with DIPLOMICS, will be

running a training workshop aimed at upskilling of researchers interested in Microbiome 16S rRNA

metabarcoding research.


The training will be focused on bacterial 16S rRNA metabacording, however, the fundamental techniques learnt can be applied to other organisms and marker genes.

LEARNING OBJECTIVES

By the conclusion of the workshop, participants will have learned about the following:

  • Understanding the mechanisms and chemistry behind Next Generation Sequencing technologies used in microbiome studies

  • General consideration in the design of sampling protocols and experimental approaches

  • Generating metabarcoding libraries and multiplexing

  • Dataset curation

  • Generation of OTUs (Operational taxonomic units)

  • Classification of sequence reads

  • Data analyses: Species diversity indices, NMDS, PCoA (using R)

PARTICIPANT FEEDBACK

No feedback from participants.

LEARNING RESOURCES

Awaiting

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